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Title:
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Evaluating Angiotensin II Type 1 Receptor Changes in Post- Renal Insufficiency and in Left Anterior Descending Artery Ligation Animal Models Using [11C]Methyl-Candesartan |
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Author:
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Mackasey, Kumiko |
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Abstract:
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Non invasive in vivo imaging will lead to better understanding of Angiotensin II Type 1 Receptor’s (AT1R) role in disease progression and may guide therapy in cardiovascular patients. Two models were used in this project: 5/6 nephrectomy and transient left anterior descending (LAD) ligation. Rats were scanned with [13N]ammonia and [11C]methyl-candesartan, both of which are Positron Emission Tomography (PET) tracers, at 8 weeks (nephrectomy) and 2 weeks (LAD ligation) after surgery. Western blot analysis was used to corroborate PET data. Nephrectomy: Renal AT1R image analysis displayed a 40% decrease in kidney AT1R in nephrectomized animals compared to sham (p<0.05) which was confirmed with Western blot and biodistribution. LAD ligation: Left Ventricle AT1R Western blot analysis exhibited a 60% increase in 20min ligation (p<0.05) with maintained myocardial blood flow. In conclusion, changes in renal AT1R were successfully imaged using [11C]methyl-candesartan in nephrectomized animals, and 20min LAD ligation/reperfusion is an appropriate model to image an increase in cardiac AT1R following ischemic injury. |
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Date:
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2012 |
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URI:
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http://hdl.handle.net/10393/20504
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Supervisor:
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DaSilva, Jean N
Beanlands, Robert |
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Faculty:
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Médecine / Medicine |
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Degree:
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MSc |
