Mechanisms of Recombinant Heat Shock Protein 27 Atheroprotection: NF-κB Signaling in Macrophages

Mechanisms of Recombinant Heat Shock Protein 27 Atheroprotection: NF-κB Signaling in Macrophages

Show simple item record

dc.contributor.author Salari, Samira
dc.date.accessioned 2012-03-05T17:42:39Z
dc.date.available 2012-03-05T17:42:39Z
dc.date.created 2012 en_US
dc.date.issued 2012-03-05
dc.identifier.uri http://hdl.handle.net/10393/20725
dc.description.abstract The O’Brien lab has demonstrated that Heat shock protein 27 (HSP27)shows attenuated expression in human coronary arteries as the degree of atherosclerosis progresses. Moreover, over-expression of HSP27 reduces atherogenesis in mice. The precise mechanism(s) for HSP27-mediated "atheroprotection" are incompletely understood. Nuclear Factor-kappaB (NF-κB) is a key signaling modulator in atherogenesis. Hence, this project sought to determine if recombinant HSP27 (rHSP27) alters NF-κB signaling to affect atheroprotection. Treatment of THP1 macrophages with rHSP27 resulted in degradation of IκBα, coincided with nuclear translocation of the p65 subunit and produced transcriptional evidence of activation of NF-κB signaling. When the transcriptional profile of THP1 macrophages treated with rHSP27 was analyzed using NF-κB-pathway-specific qRT-PCR arrays, among the regulated genes, IL-10 and GM-CSF mRNA levels were markedly increased, as were parallel translational effects observed. These data provide new mechanistic insights into the atheroprotective effects of HSP27. en_US
dc.language.iso en en_US
dc.subject HSP27 en_US
dc.subject NF-κB en_US
dc.subject Atherosclerosis en_US
dc.subject Macrophages en_US
dc.subject IL-10 en_US
dc.subject GM-CSF en_US
dc.subject qRT-PCR arrays en_US
dc.subject THP1 cells en_US
dc.title Mechanisms of Recombinant Heat Shock Protein 27 Atheroprotection: NF-κB Signaling in Macrophages en_US
dc.type Thèse / Thesis en_US
dc.faculty.department Biochimie, microbiologie et immunologie / Biochemistry, Microbiology and Immunology en_US
dc.contributor.supervisor O'Brien, Edward
dc.embargo.terms immediate en_US
dc.degree.name MSc en_US
dc.degree.level masters en_US
dc.degree.discipline Médecine / Medicine en_US

Files in this item

Files Size Format View
Salari_Samira_2012_thesis.pdf 7.647Mb application/pdf View/Open

This item appears in the following Collection(s)

Show simple item record


Contact information

Morisset Hall (map)
65 University Private
Ottawa ON Canada
K1N 6N5

Tel. 613-562-5800 (4563)
Fax 613-562-5195

ruor@uottawa.ca