Vitamin E as an index of tissue peroxidation: The effect of vitamin C deficiency and ischemia/reperfusion.

 dc.contributor.advisor Burton, G., en dc.contributor.author Pietrzak, Ewa M. en dc.date.accessioned 2009-03-23T14:12:06Z dc.date.available 2009-03-23T14:12:06Z dc.date.created 1993 en dc.date.issued 2009-03-23T14:12:06Z dc.identifier.citation Source: Masters Abstracts International, Volume: 33-02, page: 0534. en dc.identifier.isbn 9780315896925 en dc.identifier.uri http://hdl.handle.net/10393/6464 dc.description.abstract Levels and turnover of vitamin E ($\alpha$-T) were studied in guinea pigs placed for three weeks on a diet containing a scorbutic level of vitamin C and either a low level (LE group) or a high level (HE group) of hexadeuterium-labelled vitamin E (d$\sb6$-RRR-$\alpha$-T acetate). The levels of vitamin C in the ten tissues analyzed declined very rapidly at rates that were the same in both the LE and HE groups, indicating that the level of dietary vitamin E had no effect upon tissue vitamin C levels. On the vitamin C deficient diet, the total $\alpha$-T (d$\sb0$- + d$\sb6$-$\alpha$-T) declined significantly over 21 days in the HE group in two tissues with high P-450 enzyme activity and in one tissue with a high partial pressure of oxygen, whereas on a vitamin C-sufficient diet with the same concentration of vitamin E the levels of total $\alpha$-T remained steady in the same tissues. In the LE "scorbutic" group, the total $\alpha$-T declined only in heart and kidney, whereas in the vitamin C-sufficient LE group there was a decline of total $\alpha$-T in all tissues analyzed except brain. The results show that in guinea pigs, at least, vitamin C is indispensable for proper uptake of vitamin E from the gut and absorption into tissues. Changes of vitamin E levels also were studied in six anatomical regions of the brain of rats subjected to controlled ischemia/reperfusion. Analysis showed that ischemia/reperfusion caused statistically significant losses of vitamin E in all regions, except the pons-medulla, and the extent of loss correlated well with the previously determined deterioration of the blood-brain barrier in the corresponding regions. (Abstract shortened by UMI.) en dc.format.extent 145 p. en dc.publisher University of Ottawa (Canada). en dc.subject.classification Biology, Microbiology. en dc.title Vitamin E as an index of tissue peroxidation: The effect of vitamin C deficiency and ischemia/reperfusion. en dc.type M.Sc.Thesis (M.Sc.)--University of Ottawa (Canada), 1993. en

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