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Titre:
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Troglitazone Induces Extracellular Matrix and Cytoskeleton Remodeling in Mouse Collecting Duct Cells |
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Auteur:
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Corinaldi, Jaime; Nasrallah, Rania; Clark, Jordan; Paris, Genevieve; Miura, Pedro; Jasmin, Bernard J.; Hebert, Richard L. |
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Résumé:
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Peroxisome proliferator-activated receptor (PPARγ) has been shown to have a protective role in the nephron through its ability
to inhibit a transforming growth factor- (TGF-β) mediated fibrotic response. In contrast, PPARγ was also shown to induce
a mesenchymal transformation in epithelial intestinal cells. A fibrotic response in the collecting duct has only recently been
established; however, the entire collecting duct has not been fully examined. Inner medullary collecting duct cells (IMCD-K2)
and mouse cortical collecting duct cells (M1), representing the cortical and medullary collecting duct, were exposed to 5–10 μM
troglitazone for 24 hours. Troglitazone resulted in an elongated morphology, 60% decreases in E-cadherin and β-catenin, a 35%
decrease in α-catenin, and a 1.5-fold increase in fibronectin. These effects were not reversed with PPARγ antagonists or affected
with PPARγ overexpression. Our results indicate that troglitazone induced a mesenchymal-like transformation inM1 and IMCDK2
epithelial cells independently of PPARγ. |
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Date:
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2012 |
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URI:
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http://hdl.handle.net/10393/22782
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