Dlx Gene Regulation of Zebrafish GABAergic Interneuron Development

Dlx Gene Regulation of Zebrafish GABAergic Interneuron Development

Show full item record

Title: Dlx Gene Regulation of Zebrafish GABAergic Interneuron Development
Author: Ma, Wenqian
Abstract: Abstract The Dlx genes play an important role in the differentiation and migration of gamma-aminobutyric acid (GABA) interneurons of mice. GABAergic interneurons are born in the proliferative zones of the ventral telencephalon and migrate to the cortex early during mouse development. Single Dlx mutant mice show only subtle phenotypes. However, the migration of immature interneurons is blocked in the ventral telencephalon of Dlx1/Dlx2 double mutant mice leading to reduction of GABAergic interneurons in the cortex. Also, Dlx5/Dlx6 expression is almost entirely absent in the forebrain, most probably due to cross-regulatory mechanisms. In zebrafish, the role of dlx genes in GABAergic interneuron development is unknown. By injecting Morpholino, we double knocked down dlx1 and dlx2 genes in wildtype zebrafish to investigate the function of the two genes in zebrafish GABAergic interneuron development. By comparing different subsets of GABAergic interneuron development in wildtype and dlx1/2 morphant zebrafish forebrain, we found out that at 3dpf, 4dpf and 7dpf, double knockdown of dlx1 and dlx2 genes in zebrafish remarkably reduced the number of Calbindin-, Somatostatin- and Parvalbumin-positive GABAergic neurons, whereas the development of Calretinin-positive neurons is slightly affected. These results suggest that in zebrafish, dlx1a and dlx2a genes are important for the development of certain subtypes of GABAergic interneurons (Calbindin-, Somatostatin- and Parvalbumin-positive neurons) and may have minor influence on Calretinin-positive neuron development. This also suggests that different regulatory mechanisms are involved in the development of the different subtypes of GABAergic interneurons.
Date: 2011
URI: http://hdl.handle.net/10393/19970
Supervisor: Ekker, Marc
Faculty: Sciences / Science
Degree: msc

Files in this item

Files Size Format View
Ma_Wenqian_2011_thesis.pdf 3.655Mb application/pdf View/Open

This item appears in the following Collection(s)

Show full item record


Contact information

Morisset Hall (map)
65 University Private
Ottawa ON Canada
K1N 6N5

Tel. 613-562-5800 (4563)
Fax 613-562-5195

ruor@uottawa.ca